Integrated hepatic transcriptome and proteome analysis of mice with high-fat diet-induced nonalcoholic fatty liver disease

J Nutr Biochem. 2011 Jan;22(1):38-45. doi: 10.1016/j.jnutbio.2009.11.009. Epub 2010 Mar 20.


Nonalcoholic fatty liver disease (NAFLD) is the most common form of liver disease in the US and refers to a wide spectrum of liver damage, including simple steatosis, steatohepatitis, fibrosis and cirrhosis. The goal of the present study was to achieve a more detailed understanding of the molecular changes in response to high fat-induced liver steatosis through the identification of a differentially expressed liver transcriptome and proteome. Male C57/BL6 mice fed a high-fat lard diet for 8 weeks developed visceral obesity and hepatic steatosis characterized by significantly increased liver and plasma free fatty acid and triglyceride levels and plasma alanine aminotransferase activities. Transcriptome analysis demonstrated that, compared to the control diet (CD), high-fat diet changed the expression of 309 genes (132 up- and 177 down-regulated; by a twofold change and more, P<.05). Multiple genes encoding proteins involved in lipogenesis were down-regulated, whereas genes involved in fatty acid oxidation were up-regulated. Proteomic analysis revealed 12 proteins which were differentially expressed. Of these, glutathione S-transferases mu1 and pi1 and selenium-binding protein 2 were decreased at both the gene and protein levels. This is the first study to perform a parallel transcriptomic and proteomic analysis of diet-induced hepatic steatosis. Several key pathways involving xenobiotic and lipid metabolism, the inflammatory response and cell-cycle control were identified. These pathways provide targets for future mechanistic and therapeutic studies as related to the development and prevention of NAFLD.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Validation Study

MeSH terms

  • Animals
  • Dietary Fats / adverse effects
  • Fatty Acids / metabolism
  • Fatty Liver / metabolism*
  • Fatty Liver / pathology
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Glutathione S-Transferase pi / chemistry
  • Glutathione S-Transferase pi / genetics
  • Glutathione S-Transferase pi / metabolism
  • Glutathione Transferase / chemistry
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism
  • Lipogenesis
  • Liver / metabolism*
  • Liver / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Oligonucleotide Array Sequence Analysis
  • Proteome / chemistry
  • Proteome / genetics
  • Proteome / metabolism*
  • RNA, Messenger / metabolism
  • Selenium-Binding Proteins / chemistry
  • Selenium-Binding Proteins / genetics
  • Selenium-Binding Proteins / metabolism
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Tandem Mass Spectrometry
  • Two-Dimensional Difference Gel Electrophoresis


  • Dietary Fats
  • Fatty Acids
  • Proteome
  • RNA, Messenger
  • Selenbp2 protein, mouse
  • Selenium-Binding Proteins
  • Glutathione S-Transferase pi
  • Glutathione Transferase
  • Gstp1 protein, mouse
  • glutathione S-transferase M1
  • lard