DNA methylation profiling using the methylated-CpG island recovery assay (MIRA)

Methods. 2010 Nov;52(3):213-7. doi: 10.1016/j.ymeth.2010.03.004. Epub 2010 Mar 19.

Abstract

The methylated-CpG island recovery assay (MIRA) exploits the intrinsic specificity and the high affinity of a methylated-CpG-binding protein complex (MBD2B and MBD3L1) to methylated CpG dinucleotides in genomic DNA. The MIRA approach works on double-stranded DNA and does not depend on the application of methylation-sensitive restriction enzymes. It can be performed on a few hundred nanograms of genomic DNA. Recently, the MIRA technique has been used to profile DNA methylation patterns at a resolution of 100 base pairs along the entire genome of normal human B-lymphocytes. The MIRA method is compatible with microarray and next generation DNA sequencing approaches. We describe the principles and details of this method applied for methylation profiling of genomes containing methylated CpG sequences.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • CpG Islands / genetics*
  • DNA / chemistry
  • DNA / genetics*
  • DNA / metabolism*
  • DNA Methylation*
  • DNA-Binding Proteins / metabolism*
  • Genetic Techniques*
  • Humans

Substances

  • DNA-Binding Proteins
  • DNA