Analysis of hemoglobin A1c from dried blood spot samples with the Tina-quantR II immunoturbidimetric method

J Diabetes Sci Technol. 2010 Mar 1;4(2):244-9. doi: 10.1177/193229681000400203.


Background: Hemoglobin A1c (HbA1c) has been endorsed as a tool for the diagnosis of diabetes. This test requires instrumentation that may not be available in underdeveloped areas. Dried blood spot (DBS) samples collected by finger stick procedures offer a mechanism to transport samples to laboratories that do measure HbA1c.

Methods: Whole blood (ethylenediaminetetraacetic acid) was applied to Ahlstrom 226 filter paper. These DBS samples were compared to whole blood samples using the Roche Tina-quant II immunoturbidometric assay. Hemoglobin A1c stability on DBS was assessed at three temperatures-4, 25, and 40 degrees C-for up to 9 days. A 44-day study was also done for DBS at 20-25 degrees C.

Results: The Tina-quant II DBS method showed excellent agreement with whole blood HbA1c results (r(2) = 0.99) with a slight positive mean bias of 0.08 +/- 0.04% HbA1c (95% confidence interval). The variation in HbA1c on DBS samples subjected to different temperatures and times did not exceed 5.6%.

Conclusions: Dried blood spot samples represent an alternative to whole blood for HbA1c by measurement when transporting whole blood is not feasible.

MeSH terms

  • Blood Chemical Analysis / methods
  • Blood Specimen Collection / methods
  • Chromatography, High Pressure Liquid / methods
  • Diabetes Mellitus / blood*
  • Diabetes Mellitus / diagnosis
  • Drug Stability
  • Fingers / blood supply
  • Glycated Hemoglobin A / analysis*
  • Humans
  • Nephelometry and Turbidimetry / methods
  • Sensitivity and Specificity
  • Time Factors


  • Glycated Hemoglobin A