Effect of an extract of Andrographis paniculata leaves on inflammatory and allergic mediators in vitro

J Ethnopharmacol. 2010 May 27;129(2):203-7. doi: 10.1016/j.jep.2010.03.007. Epub 2010 Mar 20.


Aim of study: Andrographis paniculata has been known to possess widespread traditional application in the treatment of allergy and inflammatory diseases. In the current study, we sought to examine the effects of an extract of Andrographis paniculata leaves on inhibition of lipopolysaccharide (LPS) induced [nitric oxide (NO), prostaglandin E(2) (PGE(2)), interleukin-1beta (IL-1 beta), and interleukin-6 (IL-6)] and calcimycin (A23187) induced [leukotriene B(4) (LTB(4)), thromboxane B(2) (TXB(2)) and histamine] mediators in diverse cell based models.

Materials and methods: Effect of an extract of Andrographis paniculata leaves (AP) was studied on inhibition of LPS induced NO, PGE(2), IL-1 beta and IL-6 in J774A.1 murine macrophages; A23187 induced LTB(4) and TXB(2) in HL-60 promyelocytic leukemic cells and histamine in RBL-2H3 rat basophilic leukemia cells.

Results and conclusion: AP illustrated significant alleviation of pro-inflammatory, inflammatory, and allergic mediators. However, no inhibition was observed against histamine release. This outcome has been summed up to deduce that AP is fairly potent in attenuating the inflammation by inhibiting pro-inflammatory (NO, IL-1 beta and IL-6), inflammatory (PGE(2) and TXB(2)) and allergic (LTB(4)) mediators.

MeSH terms

  • Andrographis* / chemistry
  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology*
  • Calcimycin / pharmacology
  • Cell Line
  • Cell Line, Tumor
  • Humans
  • Hypersensitivity / immunology*
  • Inflammation Mediators / isolation & purification
  • Inflammation Mediators / metabolism*
  • Leukotriene B4 / metabolism*
  • Lipopolysaccharides
  • Macrophages / drug effects*
  • Mice
  • Plant Extracts / pharmacology*
  • Plant Leaves
  • Rats


  • Anti-Bacterial Agents
  • Anti-Inflammatory Agents
  • Inflammation Mediators
  • Lipopolysaccharides
  • Plant Extracts
  • Leukotriene B4
  • Calcimycin