Click chemistry with DNA

Chem Soc Rev. 2010 Apr;39(4):1388-405. doi: 10.1039/b901971p. Epub 2010 Feb 9.


The advent of click chemistry has led to an influx of new ideas in the nucleic acids field. The copper catalysed alkyne-azide cycloaddition (CuAAC) reaction is the method of choice for DNA click chemistry due to its remarkable efficiency. It has been used to label oligonucleotides with fluorescent dyes, sugars, peptides and other reporter groups, to cyclise DNA, to synthesise DNA catenanes, to join oligonucleotides to PNA, and to produce analogues of DNA with modified nucleobases and backbones. In this critical review we describe some of the pioneering work that has been carried out in this area (78 references).

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Alkynes / chemistry
  • Azides / chemistry
  • Catalysis
  • Copper / chemistry
  • Cyclization
  • DNA / chemistry*
  • Oligonucleotides / chemistry
  • Peptide Nucleic Acids / chemistry


  • Alkynes
  • Azides
  • Oligonucleotides
  • Peptide Nucleic Acids
  • Copper
  • DNA