Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2010 Mar;75(3):237-56.
doi: 10.1111/j.1747-0285.2009.00940.x.

NMR-based Screening of Membrane Protein Ligands

Affiliations
Review

NMR-based Screening of Membrane Protein Ligands

Naveena Yanamala et al. Chem Biol Drug Des. .

Erratum in

  • Chem Biol Drug Des. 2010 Aug;76(2):189. van Fleet, Bart [corrected to van Vliet, Bart]

Abstract

Membrane proteins pose problems for the application of NMR-based ligand-screening methods because of the need to maintain the proteins in a membrane mimetic environment such as detergent micelles: they add to the molecular weight of the protein, increase the viscosity of the solution, interact with ligands non-specifically, overlap with protein signals, modulate protein dynamics and conformational exchange and compromise sensitivity by adding highly intense background signals. In this article, we discuss the special considerations arising from these problems when conducting NMR-based ligand-binding studies with membrane proteins. While the use of (13)C and (15)N isotopes is becoming increasingly feasible, (19)F and (1)H NMR-based approaches are currently the most widely explored. By using suitable NMR parameter selection schemes independent of or exploiting the presence of detergent, (1)H-based approaches require least effort in sample preparation because of the high sensitivity and natural abundance of (1)H in both, ligand and protein. On the other hand, the (19)F nucleus provides an ideal NMR probe because of its similarly high sensitivity to that of (1)H and the lack of natural (19)F background in biologic systems. Despite its potential, the use of NMR spectroscopy is highly underdeveloped in the area of drug discovery for membrane proteins.

Similar articles

See all similar articles

Cited by 2 articles

Publication types

LinkOut - more resources

Feedback