Proteomic analysis of prostate cancer metastasis-derived prostasomes

Anticancer Res. 2010 Feb;30(2):285-90.


Background: The secretory epithelial cells of the prostate gland use sophisticated vehicles in the form of prostasomes to relay important information to sperm cells in semen. This prostasome-forming and secretory ability of the epithelial cells is also preserved in poorly differentiated prostate cancer cells. The aim of the present investigation was to conduct a proteomic analysis of metastasis-derived prostasomes.

Materials and methods: We investigated prostasomes from vertebral metastases of prostate cancer by 2-dimensional electrophoresis and matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) protein characterization.

Results: Twenty-five unique protein spots were identified by MALDI-TOF and another five proteins were determined by mass spectrometry (MS)/MS. Annexins A1, A3 and A5, as well as dimethylarginine dimethylaminohydrolase 1 were among the identified proteins. The annexins and dimethylarginine dimethylaminohydrolase 1 found in cancer-derived prostasomes can act, among others, as angiogenic factors and can increase the vascular development in the neighbourhood of the tumour.

Conclusion: Cancer-derived prostasomes may play an important role in the interaction between tumour cells and their environment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / metabolism*
  • Bone Neoplasms / metabolism*
  • Bone Neoplasms / secondary*
  • Electrophoresis, Gel, Two-Dimensional
  • Exosomes / metabolism*
  • Humans
  • Male
  • Prostatic Neoplasms / metabolism*
  • Prostatic Neoplasms / pathology
  • Proteome / analysis*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization


  • Biomarkers, Tumor
  • Proteome