The complete genome sequence of Haloferax volcanii DS2, a model archaeon

PLoS One. 2010 Mar 19;5(3):e9605. doi: 10.1371/journal.pone.0009605.


Background: Haloferax volcanii is an easily culturable moderate halophile that grows on simple defined media, is readily transformable, and has a relatively stable genome. This, in combination with its biochemical and genetic tractability, has made Hfx. volcanii a key model organism, not only for the study of halophilicity, but also for archaeal biology in general.

Methodology/principal findings: We report here the sequencing and analysis of the genome of Hfx. volcanii DS2, the type strain of this species. The genome contains a main 2.848 Mb chromosome, three smaller chromosomes pHV1, 3, 4 (85, 438, 636 kb, respectively) and the pHV2 plasmid (6.4 kb).

Conclusions/significance: The completed genome sequence, presented here, provides an invaluable tool for further in vivo and in vitro studies of Hfx. volcanii.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acids / chemistry
  • Archaea / genetics*
  • Chromosome Mapping
  • Codon
  • Computational Biology / methods
  • Gene Library
  • Genome
  • Genome, Archaeal*
  • Haloferax volcanii / genetics*
  • Isoelectric Point
  • Open Reading Frames
  • Phylogeny
  • Sequence Analysis, DNA
  • Signal Transduction


  • Amino Acids
  • Codon