Derivation and characterisation of the human embryonic stem cell line, OxF1

In Vitro Cell Dev Biol Anim. 2010 Apr;46(3-4):173-7. doi: 10.1007/s11626-010-9283-5. Epub 2010 Mar 24.


OxF1 is a human embryonic stem cell line derived from a surplus embryo donated through the Oxford IVF clinic. The cells have a stable 46 XX karyotype and show expression of Oct 4, Nanog and TRA-1-60. Embryoid bodies differentiate into cells that represent all three germ layers as demonstrated by immunohistochemical localisation of beta III tubulin, nestin, desmin, smooth muscle actin, Gata 6 and cytokeratin 18. Directed differentiation through haematopoiesis has been demonstrated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cell Line / cytology*
  • Cell Lineage
  • Embryo, Mammalian / cytology
  • Embryonic Stem Cells / cytology*
  • Germ Layers / cytology
  • Humans
  • Myeloid Cells / cytology
  • United Kingdom