The C. elegans homolog of nucleoporin Nup98 is required for the integrity and function of germline P granules

Development. 2010 May;137(9):1441-50. doi: 10.1242/dev.047654. Epub 2010 Mar 24.


C. elegans P granules are conserved cytoplasmic ribonucleoprotein complexes that are unique to the germline and essential for fertility. During most of germline development, P granules are perinuclear and associate with clusters of nuclear pores. In an RNAi screen against nucleoporins, we have identified a specific nucleoporin essential for P granule integrity and function. The C. elegans homolog of vertebrate Nup98 (CeNup98) is enriched in P granules and associates with the translationally repressed, P granule-enriched mRNA nos-2 (nanos homolog). Loss of CeNup98 causes P granules to disperse in the cytoplasm and to release nos-2 mRNA. Embryos depleted for CeNup98 express a nos-2 3'UTR reporter prematurely. In the mouse, Nup98 immunoprecipitates with the germ granule component MVH. Our findings suggest that, in germ cells, the function of Nup98 extends beyond transport at the nuclear pore to include mRNA regulation in the cytoplasm.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Blotting, Western
  • Caenorhabditis elegans / embryology*
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism*
  • DEAD-box RNA Helicases / genetics
  • DEAD-box RNA Helicases / metabolism
  • Embryo, Nonmammalian / metabolism
  • Germ Cells / metabolism*
  • Immunoprecipitation
  • In Situ Hybridization
  • Mice
  • Mice, Inbred C57BL
  • Nuclear Pore Complex Proteins / genetics
  • Nuclear Pore Complex Proteins / metabolism*
  • Protein Binding
  • RNA Interference
  • Reverse Transcriptase Polymerase Chain Reaction


  • Caenorhabditis elegans Proteins
  • Nuclear Pore Complex Proteins
  • nuclear pore complex protein 98
  • Ddx4 protein, mouse
  • DEAD-box RNA Helicases