Comparative and combined effect of chlorogenic acid and tetrahydrocurcumin on antioxidant disparities in chemical induced experimental diabetes

Mol Cell Biochem. 2010 Aug;341(1-2):109-17. doi: 10.1007/s11010-010-0442-5. Epub 2010 Mar 26.


The present study evaluates the combined effect of tetrahydrocurcumin and chlorogenic acid on oxidative stress in streptozotocin-nicotinamide-induced diabetic rats. Rats were rendered diabetic by a single intraperitoneal injection (i.p) of streptozotocin (45 mg/kg BW), 15 min after an i.p injection of nicotinamide (110 mg/kg BW). The levels of fasting plasma glucose and insulin were estimated. As an index of oxidative stress, the levels of enzymic antioxidants and lipid peroxidation products were analyzed in liver and kidney. Diabetic rats showed an increase in the levels of fasting plasma glucose, lipid peroxidative products such as thiobarbituric acid reactive substances and lipid hydroperoxides and a decrease in plasma insulin, and enzymic antioxidants viz., superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase. Combined administration of tetrahydrocurcumin (80 mg/kg BW) and chlorogenic acid (5 mg/kg BW) to diabetic rats for 45 days, reversed the biochemical changes to near normal. The above findings were supported by histological observations of the liver and kidney. Together the present study clearly reflects that combined dosage of tetrahydrocurcumin and chlorogenic acid augments enzymic antioxidants with a concomitant decrease in lipid peroxidation and protects against streptozotocin-nicotinamide-induced type 2 diabetes in experimental rats.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / analysis*
  • Chlorogenic Acid / administration & dosage
  • Chlorogenic Acid / pharmacology*
  • Curcumin / administration & dosage
  • Curcumin / analogs & derivatives*
  • Curcumin / pharmacology
  • Diabetes Mellitus, Experimental / chemically induced
  • Diabetes Mellitus, Experimental / drug therapy*
  • Diabetes Mellitus, Type 2 / drug therapy
  • Drug Combinations
  • Kidney / chemistry
  • Lipid Peroxidation / drug effects
  • Liver / chemistry
  • Niacinamide
  • Oxidative Stress / drug effects*
  • Oxidoreductases / analysis
  • Protective Agents / therapeutic use
  • Rats
  • Streptozocin
  • Treatment Outcome


  • Antioxidants
  • Drug Combinations
  • Protective Agents
  • tetrahydrocurcumin
  • Niacinamide
  • Chlorogenic Acid
  • Streptozocin
  • Oxidoreductases
  • Curcumin