The postnatal development of gut lamina propria lymphocytes: number, proliferation, and T and B cell subsets in conventional and germ-free pigs

Pediatr Res. 1991 Mar;29(3):237-42. doi: 10.1203/00006450-199103000-00004.


The lamina propria (LP) contains many lymphocytes that are effector cells as well as memory cells of the gut immune system. This compartment was studied in normal and germ-free pigs in the early postnatal period up to 91 d of age. The number of LP lymphocytes nearly doubled between the 1st and 29th d. LP lymphocytes proliferated more in the crypt region than in the villi with a mitotic rate/h comparable to that in nonfollicular compartments of Peyer's patches and lymph nodes. The determination of the subpopulations of LP lymphocytes showed a 10-fold increase in CD2+ cells between d 1 and 40. About 80% of the LP T cells in 1- and 5-d-old pigs had the unusual CD2+CD4-CD8- phenotype. Ig-positive cells appeared later in the postnatal period than the T cells. On d 1, only a few IgM+ cells were observed. In 40-d-old animals, the number of IgA+ cells exceeded that of IgM+. Ten times more Ig+ cells were detected in the crypt region than in the villi. The germ-free pigs at an age of 49 d had a T cell subset pattern comparable to that of 5-d-old normal animals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocyte Subsets / cytology
  • B-Lymphocyte Subsets / immunology
  • Cell Count
  • Cell Division
  • Female
  • Germ-Free Life
  • Immunologic Memory
  • Intestine, Small / cytology*
  • Intestine, Small / growth & development
  • Intestine, Small / immunology
  • Lymphocyte Subsets / cytology*
  • Lymphocyte Subsets / immunology
  • Male
  • Swine
  • T-Lymphocyte Subsets / cytology
  • T-Lymphocyte Subsets / immunology