cDNA-derived amino acid sequence of the NADH-binding 51-kDa subunit of the bovine respiratory NADH dehydrogenase reveals striking similarities to a bacterial NAD(+)-reducing hydrogenase

Proc Natl Acad Sci U S A. 1991 May 15;88(10):4225-9. doi: 10.1073/pnas.88.10.4225.


A lambda gt10 bovine brain and a lambda gt11 bovine heart cDNA library were screened with oligonucleotide probes corresponding to partial protein sequences directly determined from the isolated 51-kDa subunit of the bovine respiratory-chain NADH dehydrogenase. Clones were isolated that encode a protein of 464 amino acids containing all the 11 partial tryptic peptide sequences determined from the 51-kDa subunit. The size and amino acid composition of this protein agree with those determined for the purified 51-kDa subunit. Furthermore, this protein contains a putative NADH-binding domain, a possible FMN-binding site, and a putative binding site for an iron-sulfur cluster. The above evidence indicates that the cloned protein is the 51-kDa subunit or its precursor. A search for sequence similarity with proteins in the Protein Identification Resource data base has revealed that the 51-kDa subunit has 32% amino acid sequence identity with a major portion of the alpha subunit of the soluble NAD(+)-reducing hydrogenase from Alcaligenes eutrophus. In particular, there are three segments of high sequence similarity (70-88%) between the two proteins which correspond to the three ligand-binding sites.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alcaligenes / enzymology*
  • Alcaligenes / genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Brain / enzymology
  • Cattle
  • Cloning, Molecular
  • DNA / genetics
  • DNA / isolation & purification*
  • Kidney / enzymology
  • Molecular Sequence Data
  • Molecular Weight
  • Myocardium / enzymology
  • NAD / metabolism*
  • NADH Dehydrogenase / chemistry*
  • NADH Dehydrogenase / genetics
  • Oligonucleotide Probes
  • Oxidoreductases / chemistry*
  • Oxidoreductases / genetics
  • RNA, Messenger / analysis
  • Sequence Homology, Nucleic Acid
  • Tissue Distribution


  • Oligonucleotide Probes
  • RNA, Messenger
  • NAD
  • DNA
  • Oxidoreductases
  • hydrogen dehydrogenase
  • NADH Dehydrogenase

Associated data

  • GENBANK/M63009
  • GENBANK/M64435
  • GENBANK/M65272
  • GENBANK/M68846
  • GENBANK/M68847
  • GENBANK/M68848
  • GENBANK/M68849
  • GENBANK/M68850
  • GENBANK/M68851
  • GENBANK/M68852