Seed-expressed casein kinase I acts as a positive regulator of the SeFAD2 promoter via phosphorylation of the SebHLH transcription factor

Plant Mol Biol. 2010 Jul;73(4-5):425-37. doi: 10.1007/s11103-010-9630-7. Epub 2010 Mar 28.


Microsomal oleic acid desaturase (FAD2) catalyzes the first committed step of the biosynthesis of polyunsaturated fatty acids via extra-plastidial desaturation of oleic acid to linoleic acid. In the regulatory mechanism controlling seed-specific SeFAD2 expression, trans-activation of the seed-specific SeFAD2 promoter is mediated by the SebHLH transcription factor (Kim et al. in Plant Mol Biol 64:453-466, 2007). In this study, a protein interacting with SebHLH was isolated from yeast two-hybrid analysis. The protein shares approximately 80% sequence identity with other putative casein kinases and was named SeCKI (Sesame Casein Kinase I). SeCKI transcripts were predominantly expressed in developing sesame seeds and were induced approximately threefold by exogenous application of ABA. eGFP:SeCKI fusion protein was localized to the nucleus. The SeCKI protein specifically bound to SebHLH. The SeCKI protein was autophosphorylated in a calcium-independent manner and transphosphorylated the SebHLH protein. Both the SebHLH and the SeCKI genes or both the SebHLH and mutated SemCKI (K182G) genes, under the control of CaMV 35S promoter, and the GUS reporter gene driven by SeFAD2 promoter containing E- and G-Box motifs were co-expressed in developing sesame seeds. This co-expression revealed that SeCKI enhanced the SebHLH-mediated transactivation of the SeFAD2 gene promoter via phosphorylation of the SebHLH transcription factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / metabolism*
  • Calcium / pharmacology
  • Casein Kinase I / chemistry
  • Casein Kinase I / genetics
  • Casein Kinase I / metabolism*
  • Cell Nucleus / drug effects
  • Cell Nucleus / enzymology
  • Fatty Acid Desaturases / genetics*
  • Gene Expression Regulation, Developmental / drug effects
  • Gene Expression Regulation, Plant / drug effects
  • Green Fluorescent Proteins / metabolism
  • Molecular Sequence Data
  • Organ Specificity / drug effects
  • Organ Specificity / genetics
  • Phosphorylation / drug effects
  • Promoter Regions, Genetic*
  • Protein Binding / drug effects
  • Protein Transport / drug effects
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / metabolism
  • Seeds / drug effects
  • Seeds / enzymology*
  • Seeds / genetics*
  • Sesamum / drug effects
  • Sesamum / embryology
  • Sesamum / enzymology*
  • Sesamum / genetics
  • Transcription, Genetic / drug effects
  • Two-Hybrid System Techniques


  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Fatty Acid Desaturases
  • delta-12 fatty acid desaturase
  • Casein Kinase I
  • Calcium

Associated data

  • GENBANK/GQ444335