Na(+):aspartate coupling stoichiometry in the glutamate transporter homologue Glt(Ph)

Biochemistry. 2010 May 4;49(17):3511-3. doi: 10.1021/bi100430s.

Abstract

The Na(+) aspartate symporter Glt(Ph) from Pyrococcus horikoshii is the only member of the glutamate transporter family for which crystal structures have been determined. The cation:aspartate coupling stoichiometry is unknown, thus hampering the elucidation of the ion coupling mechanism. Here we measure transport of (22)Na(+) and [(14)C]aspartate in proteoliposomes containing purified Glt(Ph) and demonstrate that three Na(+) ions are symported with aspartate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Transport System X-AG / chemistry*
  • Aspartic Acid / chemistry*
  • Aspartic Acid / metabolism
  • Biological Transport
  • Protein Conformation
  • Proteolipids / chemistry
  • Pyrococcus horikoshii / chemistry
  • Sodium / chemistry*
  • Sodium / metabolism

Substances

  • Amino Acid Transport System X-AG
  • Proteolipids
  • proteoliposomes
  • Aspartic Acid
  • Sodium