Abstract
Human mitochondrial transcription factor 1 (mtTF1) has been sequenced and is a nucleus-encoded DNA binding protein of 204 amino acids (24,400 daltons). Expression of human mtTF1 in bacteria yields a protein with correct physical properties and the ability to activate mitochondrial DNA promoters. Analysis of the protein's sequence reveals no similarities to any other DNA binding proteins except for the existence of two domains that are characteristic of high mobility group (HMG) proteins. Human mtTF1 is most closely related to a DNA binding HMG-box region in hUBF, a human protein known to be important for transcription by RNA polymerase I.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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DNA, Mitochondrial / genetics*
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DNA-Binding Proteins / genetics*
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Gene Library
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High Mobility Group Proteins / genetics*
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Humans
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Mitochondria / metabolism*
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Mitochondrial Proteins*
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Molecular Sequence Data
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Molecular Weight
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Nuclear Proteins*
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Nucleic Acid Hybridization
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Oligonucleotide Probes
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Open Reading Frames
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Promoter Regions, Genetic
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RNA, Messenger / genetics
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RNA, Messenger / isolation & purification
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Sequence Homology, Nucleic Acid
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Transcription Factors / genetics*
Substances
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DNA, Mitochondrial
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DNA-Binding Proteins
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High Mobility Group Proteins
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Mitochondrial Proteins
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Nuclear Proteins
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Oligonucleotide Probes
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RNA, Messenger
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TFAM protein, human
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Transcription Factors
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mitochondrial transcription factor A
Associated data
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GENBANK/M62810
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GENBANK/M64179
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GENBANK/M64180
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GENBANK/M64181
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GENBANK/M64182
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GENBANK/M64183
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GENBANK/M64184
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GENBANK/M64185
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GENBANK/M64186
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GENBANK/M64187