Increased 18F-FDG uptake and expression of Glut1 in the EMT transformed breast cancer cells induced by TGF-beta

Neoplasma. 2010;57(3):234-40. doi: 10.4149/neo_2010_03_234.

Abstract

As carcinomas progress, the tumors may lose epithelial morphology and acquire mesenchymal characteristics typically called epithelial-mesenchymal transition (EMT), which is commonly associated with increased cell migration, enables cells to dissociate from their original tissue and form metastasis in distant organs. In addition to molecular and morphologic changes, the EMT transformed cells also showed the change of sensitivity to chemotherapeutics. In order to detect the EMT transition in vivo clinically, we detected the change of metabolism of MCF-7 cells after being induced by TGF-beta to form EMT condition by MTT and 18F-FDG uptake.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / chemistry
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology
  • Cadherins / analysis
  • Cell Line, Tumor
  • Cell Movement
  • Cell Transformation, Neoplastic*
  • Epithelial Cells / pathology*
  • Female
  • Fluorodeoxyglucose F18 / pharmacokinetics*
  • Glucose Transporter Type 1 / analysis*
  • Humans
  • Mesoderm / pathology*
  • Radiopharmaceuticals / pharmacokinetics*
  • Transforming Growth Factor beta / pharmacology*
  • Vimentin / analysis

Substances

  • Cadherins
  • Glucose Transporter Type 1
  • Radiopharmaceuticals
  • SLC2A1 protein, human
  • Transforming Growth Factor beta
  • Vimentin
  • Fluorodeoxyglucose F18