Characterisation of a haemagglutinin from Hokkaido red bean (Phaseolus vulgaris cv. Hokkaido red bean)

J Sci Food Agric. 2010 Jan 15;90(1):70-7. doi: 10.1002/jsfa.3782.

Abstract

Background: A haemagglutinin was purified from Japanese Hokkaido red beans (Phaseolus vulgaris cv. Hokkaido red bean) with a procedure that included three chromatographic media.

Results: Haemagglutinating activity was adsorbed on DEAE cellulose, Affi-gel blue gel and Mono S. The pure haemagglutinin was a homodimer and each subunit was around 30 kDa in molecular mass. The haemagglutinating activity of this agglutinin could not be inhibited by a variety of simple sugars at 200 mmol L(-1) concentration including alpha-L-fucose, D(+)-galactose, D(+)-glucose, D(+)-glucosamine, D(-)galactosamine, galacturonic acid, (+)-lactose, D(+)-melibose, L(-)-mannose, D(+)-mannose, D-mannosamine, D(+)-raffinose, L-rhamnose, (+)-xylose and galacturonic acid. The haemagglutinating activity was fully retained at pH 4-11 and at 0-80 degrees C, but was completely lost at extreme pH values (0-2 and 13-14) and at very high temperatures (90 degrees C and 100 degrees C). The haemagglutinin exhibited a weak mitogenic activity toward mouse splenocytes, a stronger anti-proliferative activity than Con A toward HepG2 (human hepatoma) cells and inhibited >80% of HIV-1 reverse transcriptase inhibitory activity at 3.3 micromol L(-1). It was devoid of anti-fungal activity.

Conclusion: Hokkaido red bean haemagglutinin possesses a potent anti-proliferative effect on HepG2 cells.

MeSH terms

  • Adsorption
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / isolation & purification
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Carbohydrates
  • Carcinoma, Hepatocellular / drug therapy*
  • Cell Proliferation / drug effects*
  • Chromatography / methods
  • Concanavalin A
  • DEAE-Cellulose
  • HIV Reverse Transcriptase / antagonists & inhibitors
  • Hep G2 Cells
  • Hot Temperature
  • Humans
  • Hydrogen-Ion Concentration
  • Mitogens / chemistry
  • Mitogens / isolation & purification
  • Mitogens / pharmacology*
  • Molecular Structure
  • Phaseolus / chemistry*
  • Phytohemagglutinins / chemistry
  • Phytohemagglutinins / isolation & purification
  • Phytohemagglutinins / pharmacology*
  • Reverse Transcriptase Inhibitors / chemistry
  • Reverse Transcriptase Inhibitors / isolation & purification
  • Reverse Transcriptase Inhibitors / pharmacology*
  • Spleen / cytology
  • Spleen / drug effects

Substances

  • Antineoplastic Agents, Phytogenic
  • Carbohydrates
  • Mitogens
  • Phytohemagglutinins
  • Reverse Transcriptase Inhibitors
  • Concanavalin A
  • DEAE-Cellulose
  • reverse transcriptase, Human immunodeficiency virus 1
  • HIV Reverse Transcriptase