Identifications of inhibitors of IgE production by human lymphocytes isolated from 'Cha Chuukanbohon Nou 6' tea leaves

J Sci Food Agric. 2010 Jan 15;90(1):168-74. doi: 10.1002/jsfa.3807.

Abstract

Background: Tea (Camellia sinensis L.) is consumed all over the world and in especially large quantities in Japan and China, where it has been used not only as a daily beverage but also for medicinal purposes for thousands of years. Tea has been found to exhibit various bioregulatory activities, including antiallergic, anticarcinogenic, antimetastatic, antioxidative, antihypertensive, antihypercholesterolemic, anti-dental caries and antibacterial effects, and to influence intestinal flora.

Results: Cha Chuukanbohon Nou 6 is a tea cultivar improved by the National Institute of Vegetable and Tea Science (NIVTS) in Japan. On comparing chemical constituents of 11 varieties of tea leaves by high-performance liquid chromatography, we found two new major compounds in Cha Chuukanbohon Nou 6. Nuclear magnetic resonance spectroscopy revealed these compounds to be theogallin and 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-beta-D-glucopyranose. The two were similar in chemical structure to strictinin, an inhibitor of immunoglobulin (Ig) production. Thus their effects on the production of Igs by peripheral blood lymphocytes were tested. Both compounds, like strictinin, inhibited IgE production.

Conclusion: The results suggest Cha Chuukanbohon Nou 6 to be the basis of an antiallergic beverage.

MeSH terms

  • Antibody Formation / drug effects
  • Blood / immunology
  • Camellia sinensis / chemistry*
  • Camellia sinensis / genetics
  • Chromatography, High Pressure Liquid
  • Gallic Acid / analogs & derivatives*
  • Gallic Acid / chemistry
  • Gallic Acid / isolation & purification
  • Gallic Acid / pharmacology
  • Genotype
  • Humans
  • Hydrolyzable Tannins / chemistry
  • Hydrolyzable Tannins / isolation & purification*
  • Hydrolyzable Tannins / pharmacology
  • Immunoglobulin E / biosynthesis*
  • Immunosuppressive Agents / chemistry
  • Immunosuppressive Agents / isolation & purification*
  • Immunosuppressive Agents / pharmacology
  • Lymphocytes / drug effects*
  • Lymphocytes / metabolism
  • Molecular Structure
  • Phenols / metabolism
  • Plant Extracts / chemistry*
  • Plant Extracts / pharmacology
  • Plant Leaves / chemistry
  • Plant Leaves / genetics
  • Quinic Acid / analogs & derivatives*
  • Quinic Acid / chemistry
  • Quinic Acid / isolation & purification
  • Quinic Acid / pharmacology
  • Tea / chemistry

Substances

  • 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-beta-D-glucopyranose
  • Hydrolyzable Tannins
  • Immunosuppressive Agents
  • Phenols
  • Plant Extracts
  • Tea
  • strictinin
  • theogallin
  • Quinic Acid
  • Immunoglobulin E
  • Gallic Acid