Sustained release of vancomycin from a new biodegradable glue to prevent methicillin-resistant Staphylococcus aureus graft infection

Interact Cardiovasc Thorac Surg. 2010 Jul;11(1):52-5. doi: 10.1510/icvts.2010.232447. Epub 2010 Apr 1.


Prosthetic graft infection with methicillin-resistant Staphylococcus aureus (MRSA) is one of the most serious complications of cardiovascular surgery. Seeking to prevent graft infection, we evaluated the efficacy of a new biodegradable hydrogel glue (new-glue) composed of aldehyded dextran and epsilon-poly(L-lysine) which acts as a local sustained-release carrier of vancomycin. Rats (n=40) were implanted with 1-cm(2) Dacron grafts in the subcutaneous pockets. Groups (n=10 each) were as follows: no treatment (group A), topical vancomycin solution (group B), new-glue without vancomycin (group C) or new-glue containing 1 mg of vancomycin (group D). Twenty-four h after the implantation, 2.0x10(7) colony-forming units of MRSA was inoculated onto the graft surface. Seven days thereafter, the graft was sampled and cultured. The quantity of MRSA was significantly lower in group D than in the other groups (P<0.0001). About 95% of the total vancomycin was released from the new-glue over the 72 h experimental period, and the tissue concentration of vancomycin remained above the minimum inhibitory concentration for the MRSA strain throughout the experiment. This new vancomycin-containing glue effectively prevented prosthetic graft infection and thus may be a promising biodegradable drug vehicle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Topical
  • Animals
  • Anti-Bacterial Agents / administration & dosage*
  • Anti-Bacterial Agents / pharmacokinetics
  • Blood Vessel Prosthesis / adverse effects*
  • Blood Vessel Prosthesis Implantation / adverse effects*
  • Blood Vessel Prosthesis Implantation / instrumentation
  • Colony Count, Microbial
  • Delayed-Action Preparations
  • Disease Models, Animal
  • Drug Carriers*
  • Drug Compounding
  • Male
  • Methicillin-Resistant Staphylococcus aureus / drug effects*
  • Methicillin-Resistant Staphylococcus aureus / growth & development
  • Microbial Sensitivity Tests
  • Polyethylene Terephthalates
  • Prosthesis Design
  • Prosthesis-Related Infections / microbiology
  • Prosthesis-Related Infections / prevention & control*
  • Rats
  • Rats, Wistar
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / prevention & control*
  • Tissue Adhesives*
  • Tissue Distribution
  • Vancomycin / administration & dosage*
  • Vancomycin / pharmacokinetics


  • Anti-Bacterial Agents
  • Delayed-Action Preparations
  • Drug Carriers
  • Polyethylene Terephthalates
  • Tissue Adhesives
  • Vancomycin