Drosophila macrophage preparation and screening

Cold Spring Harb Protoc. 2010 Apr;2010(4):pdb.prot5404. doi: 10.1101/pdb.prot5404.

Abstract

Drosophila plasmatocytes, also known as macrophages, are part of the Drosophila innate immune system and also have roles during development. In late-stage embryos, it is possible to image macrophage migration in situ during development and when they converge at sites of wounding. This protocol describes the isolation of macrophages from third instar Drosophila larvae. The macrophages can be cultured for several hours, and fluorescently labeled macrophages can be screened using a fluorescence-imaging system.

MeSH terms

  • Animals
  • Cell Separation / methods*
  • Drosophila / immunology*
  • Macrophages*
  • Mass Screening
  • Microscopy, Fluorescence
  • Staining and Labeling