Since 1986, the Healthcare Infection Control Practices Advisory Committee (HICPAC) and the Advisory Committee on Immunization Practices (ACIP) have recommended that all health-care personnel (HCP) be vaccinated annually for influenza. Since 1989, overall influenza vaccination coverage among HCP has never exceeded 49% in any season, according to estimates from the National Health Interview Survey. In August 2009, ACIP recommended that HCP be one of five initial target groups to receive the influenza A (H1N1) 2009 monovalent vaccine when it first became available. This report summarizes results of a population-based panel survey administered via the Internet during January 2010 to a nationally representative sample of 1,417 HCP to assess vaccination coverage. By mid-January 2010, estimated vaccination coverage among HCP was 37.1% for 2009 pandemic influenza A (H1N1) and 61.9% for seasonal influenza. Overall, 64.3% received either of these influenza vaccines, higher coverage than any previous season, but only 34.7% of HCP reported receiving both vaccines. The existence of an employer requirement for vaccination at the facility where the respondent was employed was associated with an eightfold greater likelihood of 2009 H1N1 vaccination compared with respondents employed by facilities with neither requirement nor recommendations; likewise, the existence of a recommendation for vaccination was associated with a fourfold greater probability of 2009 H1N1 vaccination. Health-care administrators should consider influenza vaccination coverage among employees an important measure of patient safety and make appropriate efforts to increase coverage in future seasons.