Super-SILAC mix for quantitative proteomics of human tumor tissue

Nat Methods. 2010 May;7(5):383-5. doi: 10.1038/nmeth.1446. Epub 2010 Apr 4.


We describe a method to accurately quantify human tumor proteomes by combining a mixture of five stable-isotope labeling by amino acids in cell culture (SILAC)-labeled cell lines with human carcinoma tissue. This generated hundreds of thousands of isotopically labeled peptides in appropriate amounts to serve as internal standards for mass spectrometry-based analysis. By decoupling the labeling from the measurement, this super-SILAC method broadens the scope of SILAC-based proteomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Astrocytoma / chemistry
  • Breast Neoplasms / chemistry*
  • Carbon Isotopes
  • Cell Line, Tumor
  • Female
  • Glioblastoma / chemistry
  • Humans
  • Isotope Labeling / methods*
  • Neoplasm Proteins / analysis*
  • Nitrogen Isotopes
  • Proteomics / methods*


  • Carbon Isotopes
  • Neoplasm Proteins
  • Nitrogen Isotopes