Isolation of bacteria from mouse caecal samples and description of Bacteroides sartorii sp. nov

Arch Microbiol. 2010 Jun;192(6):427-35. doi: 10.1007/s00203-010-0568-6. Epub 2010 Apr 6.


Caecal samples from wild-type and TNF(deltaARE) mice were cultured on selective media containing bile salts, amino acids or casein macro-peptides. Twenty-two strains were isolated and identified by 16S rRNA gene sequencing. Twenty-one strains showed >98% similarity to known bacteria (Blautia spp., Clostridium innocuum, Enterococcus spp., Escherichia coli, Lactobacillus murinus, Parabacteroides goldsteinii and Shigella dysenteriae). One additional isolate, strain A-C2-0, was a new bacterium. The closest relatives were Bacteroides massiliensis, Bacteroides dorei and Bacteroides vulgatus (< or = 94% similarity). Strain A-C2-0 is a Gram-negative rod that does not form spores and has a G + C content of DNA of 41.5%. Its major cellular fatty acid is C(15:0 ANTEISO), and its major respiratory quinone is MK-9. Cells are aerotolerant but grow only under strict anoxic conditions. They are resistant to cefotaxime and tobramycin. When compared with related Bacteroides spp., the new bacterium was positive for alpha-arabinosidase, negative for glutamyl glutamic acid arylamidase and did not metabolise galactose, glucose, fructose, mannose, raffinose and sucrose. Strain A-C2-0 therefore merits recognition as a member of a novel species within the genus Bacteroides, for which the name Bacteroides sartorii is proposed. The type strain is A-C2-0(T) (= DSM 21941(T) = CCUG 57211(T)).

MeSH terms

  • Animals
  • Bacteroides / classification*
  • Bacteroides / genetics
  • Bacteroides / isolation & purification*
  • Bacteroides / physiology
  • Base Composition
  • Cecum / microbiology*
  • Culture Media
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Genes, Bacterial
  • Genes, rRNA
  • Genotype
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Phenotype
  • Phylogeny
  • Sequence Analysis, DNA


  • Culture Media
  • DNA, Bacterial
  • Fatty Acids