Functional subpopulations of individual pancreatic B-cells in culture

Endocrinology. 1991 Jun;128(6):3193-8. doi: 10.1210/endo-128-6-3193.


In the present study we have used a reverse hemolytic plaque assay and frequency distribution analysis to characterize the insulin secretory response of individual rat pancreatic B-cells in culture. Heterogeneous secretory behavior was observed with a multimodal distribution, suggesting the existence of B-cell subpopulations. Insulin secretion in response to increased extracellular glucose involved both amplification of secretion by individual cells as well as recruitment of previously silent B-cells.

MeSH terms

  • Animals
  • Cells, Cultured
  • Cricetinae
  • Culture Media
  • Glucose
  • Hemolytic Plaque Technique
  • Insulin / metabolism
  • Insulin Secretion
  • Islets of Langerhans / metabolism*
  • Osmolar Concentration


  • Culture Media
  • Insulin
  • Glucose