Dynamic and static interactions between p120 catenin and E-cadherin regulate the stability of cell-cell adhesion

Cell. 2010 Apr 2;141(1):117-28. doi: 10.1016/j.cell.2010.01.017.

Abstract

The association of p120 catenin (p120) with the juxtamembrane domain (JMD) of the cadherin cytoplasmic tail is critical for the surface stability of cadherin-catenin cell-cell adhesion complexes. Here, we present the crystal structure of p120 isoform 4A in complex with the JMD core region (JMD(core)) of E-cadherin. The p120 armadillo repeat domain contains modular binding pockets that are complementary to electrostatic and hydrophobic properties of the JMD(core). Single-residue mutations within the JMD(core)-binding site of p120 abolished its interaction with E- and N-cadherins in vitro and in cultured cells. These mutations of p120 enabled us to clearly differentiate between N-cadherin-dependent and -independent steps of neuronal dendritic spine morphogenesis crucial for synapse development. NMR studies revealed that p120 regulates the stability of cadherin-mediated cell-cell adhesion by associating with the majority of the JMD, including residues implicated in clathrin-mediated endocytosis and Hakai-dependent ubiquitination of E-cadherin, through its discrete "dynamic" and "static" binding sites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cadherins / chemistry*
  • Cadherins / genetics
  • Cadherins / metabolism*
  • Catenins / chemistry*
  • Catenins / genetics
  • Catenins / metabolism*
  • Cell Adhesion*
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Humans
  • Mice
  • Models, Molecular
  • Protein Interaction Domains and Motifs
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism

Substances

  • Cadherins
  • Catenins
  • Recombinant Fusion Proteins
  • delta catenin

Associated data

  • PDB/3L6X
  • PDB/3L6Y