Extraction of polysaccharide from Ganoderma lucidum and its immune enhancement activity

Int J Biol Macromol. 2010 Oct 1;47(3):336-41. doi: 10.1016/j.ijbiomac.2010.03.019. Epub 2010 Apr 4.


In the present study, in order to maximize the yield of polysaccharides from Ganoderma lucidum, response surface methodology was employed to optimize the ultrasonic/microwave-assisted extraction (UMAE) conditions. The results indicated that the optimal extraction conditions were ultrasonic power of 50W, microwave power of 284W, extraction time of 701s and water/solid ratio of 11.6:1, respectively. Using UMAE, the yield of polysaccharides was 115.56% above that of classical hot water extraction (HWE) and increased by 27.7% as against ultrasound-assisted extraction (UAE), which confirmed the great potential application of UMAE technology in the extraction of polysaccharides. The immunological assays results demonstrated that polysaccharides of G. lucidum extracted by ultrasonic/microwave (UMP) could improve the weight of immune organ of immunocompromised mice, restore delayed-type hypersensitivity (DTH) reaction to DFNB, improve hemolysis antibody level and natural killer cell activity at high-dose. However, UMP had no noticeable effects on phagocytosis of monocyte at the tested dosage range.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbon / metabolism
  • Chemical Fractionation / methods*
  • Hemolysin Proteins / blood
  • Hot Temperature
  • Hypersensitivity, Delayed / immunology
  • Immunologic Factors / immunology*
  • Immunologic Factors / isolation & purification*
  • Killer Cells, Natural / immunology
  • Male
  • Mice
  • Microwaves
  • Polysaccharides / immunology*
  • Polysaccharides / isolation & purification*
  • Reishi / chemistry*
  • Spleen / immunology
  • Thymus Gland / immunology
  • Ultrasonics
  • Water / chemistry


  • Hemolysin Proteins
  • Immunologic Factors
  • Polysaccharides
  • Water
  • Carbon