Pup grows up: in vitro characterization of the degradation of pupylated proteins

EMBO J. 2010 Apr 7;29(7):1163-4. doi: 10.1038/emboj.2010.40.


By reconstituting the recently discovered prokaryotic ubiquitin-like protein (Pup)--proteasome degradation system in vitro, Weber-Ban and colleagues (Striebel et al, 2010) elucidate its mechanism and describe a surprising variation on the established principles of protease targeting. Nevertheless, their findings suggest that the bacterial and eukaryotic systems follow the same overall principles even if the details differ.

Publication types

  • Comment
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / metabolism*
  • Bacterial Proteins / metabolism*
  • Mycobacterium tuberculosis / metabolism*
  • Proteasome Endopeptidase Complex / metabolism*
  • Ubiquitins / metabolism*


  • Bacterial Proteins
  • Ubiquitins
  • Proteasome Endopeptidase Complex
  • Adenosine Triphosphatases
  • Mpa protein, Mycobacterium tuberculosis