Development and evaluation of the polymerase chain reaction to detect Mycoplasma genitalium

FEMS Microbiol Lett. 1991 Jan 15;61(2-3):199-203. doi: 10.1016/0378-1097(91)90551-k.


The polymerase chain reaction (PCR) was developed to detect Mycoplasma genitalium. Oligonucleotide primers were used to amplify a 374 bp region of the attachment protein of the mycoplasma. DNA from three strains of M. genitalium tested gave a characteristic PCR product which was not seen with DNA from any other source. As little as 10(-15) g of M. genitalium DNA could be detected and it was found in the vagina of progesterone-treated BALB/c mice inoculated with M. genitalium organisms later than they could be cultured from this site, but not in mice that never became colonised vaginally.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Bacteriological Techniques
  • Base Sequence
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification*
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Mycoplasma / genetics
  • Mycoplasma / isolation & purification*
  • Mycoplasma Infections / diagnosis*
  • Mycoplasma Infections / microbiology
  • Oligonucleotide Probes
  • Polymerase Chain Reaction / methods*
  • Progesterone / pharmacology
  • Vagina / drug effects
  • Vagina / microbiology*
  • Vaginal Diseases / diagnosis
  • Vaginal Diseases / microbiology


  • DNA, Bacterial
  • Oligonucleotide Probes
  • Progesterone