Nitrogen-doped graphene and its application in electrochemical biosensing

ACS Nano. 2010 Apr 27;4(4):1790-8. doi: 10.1021/nn100315s.


Chemical doping with foreign atoms is an effective method to intrinsically modify the properties of host materials. Among them, nitrogen doping plays a critical role in regulating the electronic properties of carbon materials. Recently, graphene, as a true two-dimensional carbon material, has shown fascinating applications in bioelectronics and biosensors. In this paper, we report a facile strategy to prepare N-doped graphene by using nitrogen plasma treatment of graphene synthesized via a chemical method. Meanwhile, a possible schematic diagram has been proposed to detail the structure of N-doped graphene. By controlling the exposure time, the N percentage in host graphene can be regulated, ranging from 0.11 to 1.35%. Moreover, the as-prepared N-doped graphene has displayed high electrocatalytic activity for reduction of hydrogen peroxide and fast direct electron transfer kinetics for glucose oxidase. The N-doped graphene has further been used for glucose biosensing with concentrations as low as 0.01 mM in the presence of interferences.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aspergillus niger / enzymology
  • Biosensing Techniques / methods*
  • Carbon / chemistry*
  • Catalysis
  • Electrochemistry
  • Electrodes
  • Electron Transport
  • Glucose / analysis
  • Glucose Oxidase / chemistry
  • Glucose Oxidase / metabolism
  • Kinetics
  • Models, Molecular
  • Molecular Conformation
  • Nitrogen / chemistry*


  • Carbon
  • Glucose Oxidase
  • Glucose
  • Nitrogen