Two closely related subclass II SnRK2 protein kinases cooperatively regulate drought-inducible gene expression

Plant Cell Physiol. 2010 May;51(5):842-7. doi: 10.1093/pcp/pcq041. Epub 2010 Apr 7.

Abstract

The subclass III group of SNF1-related protein kinase 2 (SnRK2) members is known to play an important role in ABA and osmotic stress signaling in Arabidopsis; however, the roles of other subclasses remain elusive. Here, we established a double mutant of SRK2C/SnR2.8 and SRK2F/SnRK2.7 to investigate the functions of subclass II SnRK2s. Microarray analysis suggested that subclass II SnRK2s regulate some drought-responsive genes involving ABA-responsive element binding transcription factors (AREB/ABF) and their targets, and quantitative reverse transcription- PCR confirmed that those genes were down-regulated significantly in srk2cf. This study indicates that subclass II SnRK2s also play important roles in drought stress signaling in Arabidopsis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Droughts*
  • Gene Expression Regulation, Plant
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Phosphorylation
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism*
  • RNA, Plant / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stress, Physiological

Substances

  • Arabidopsis Proteins
  • RNA, Plant
  • SnRK2 protein, Arabidopsis
  • Protein-Serine-Threonine Kinases