Wide field-of-view lens-free fluorescent imaging on a chip

Lab Chip. 2010 Apr 7;10(7):824-7. doi: 10.1039/b926561a. Epub 2010 Feb 25.


We demonstrate an on-chip fluorescent detection platform that can simultaneously image fluorescent micro-objects or labeled cells over an ultra-large field-of-view of 2.5 cm x 3.5 cm without the use of any lenses, thin-film filters and mechanical scanners. Such a wide field-of-view lensless fluorescent imaging modality, despite its limited resolution, might be very important for high-throughput screening applications as well as for detection and counting of rare cells within large-area microfluidic devices.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cells
  • Fluorescent Dyes*
  • Humans
  • Leukocytes / cytology
  • Leukocytes / pathology
  • Microfluidic Analytical Techniques* / instrumentation
  • Microfluidic Analytical Techniques* / methods


  • Fluorescent Dyes