Resveratrol protects against experimental stroke: putative neuroprotective role of heme oxygenase 1

Exp Neurol. 2010 Jul;224(1):325-9. doi: 10.1016/j.expneurol.2010.03.032. Epub 2010 Apr 8.

Abstract

Epidemiological and experimental reports have linked mild-to-moderate wine and/or grape consumption to a lowered incidence of cardiovascular, cerebrovascular, and peripheral vascular risk. This study revealed that resveratrol, an enriched bioactive polyphenol in red wine, selectively induces heme oxygenase 1 (HO1) in a dose- and time-dependent manner in cultured mouse cortical neuronal cells and provides neuroprotection from free-radical or excitotoxicity damage. This protection was lost when cells were treated with a protein synthesis or heme oxygenase inhibitor, suggesting that HO1 induction is at least partially required for resveratrol's prophylactic properties. Furthermore, resveratrol pretreatment dose-dependently protected mice subjected to an optimized ischemic-reperfusion stroke model. Mice in which HO1 was selectively deleted lost most, if not all, of the beneficial effects. Together, the data suggest a potential intracellular pathway by which resveratrol can provide cell/organ resistance against neuropathological conditions.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Blotting, Western
  • Cerebral Cortex / drug effects*
  • Cerebral Cortex / metabolism
  • Dose-Response Relationship, Drug
  • Heme Oxygenase-1 / genetics
  • Heme Oxygenase-1 / metabolism*
  • Infarction, Middle Cerebral Artery / drug therapy*
  • Infarction, Middle Cerebral Artery / metabolism
  • Mice
  • Mice, Knockout
  • Neurons / drug effects
  • Neurons / metabolism*
  • Neuroprotective Agents / pharmacology
  • Resveratrol
  • Statistics, Nonparametric
  • Stilbenes / pharmacology*
  • Time Factors

Substances

  • Neuroprotective Agents
  • Stilbenes
  • Heme Oxygenase-1
  • Resveratrol