Phycicoccus ginsenosidimutans sp. nov., isolated from soil of a ginseng field

Int J Syst Evol Microbiol. 2011 Mar;61(Pt 3):524-528. doi: 10.1099/ijs.0.022137-0. Epub 2010 Apr 9.

Abstract

A Gram-positive, non-motile, non-spore-forming, aerobic, coccoid-shaped bacterium, designated BXN5-13(T), was isolated from the soil of a ginseng field from Baekdu Mountain in Jilin district, China. Strain BXN5-13(T) grew optimally at 30 °C and pH 6.5-7.5 with 0-2 % (w/v) NaCl. Strain BXN5-13(T) had β-glucosidase activity that was connected with ginsenoside-converting ability, so that it was able to convert ginsenoside Rb(1) to ginsenoside F2. On the basis of 16S rRNA gene sequence analysis, the closest phylogenetic relatives of strain BXN5-13(T) were Phycicoccus aerophilus 5516T-20(T) (98.4 % 16S rRNA gene sequence similarity), P. bigeumensis MSL-03(T) (98.3 %), P. dokdonensis DS-8(T) (97.9 %) and P. jejuensis KSW2-15(T) (96.9 %). Lower sequence similarity (<97.0 %) was found with the type strains of other recognized species of the family Intrasporangiaceae. The predominant quinone was MK-8(H₄). The major fatty acids (>10 %) were iso-C₁₅:₀, C₁₇:₀, anteiso-C₁₅:₀ and iso-C₁₆:₀. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The chemotaxonomic data and the high genomic DNA G+C content of strain BXN5-13(T) (70.8l %) supported its affiliation with the genus Phycicoccus. DNA-DNA relatedness between strain BXN5-13(T) and its closest phylogenetic neighbours was below 16 %. Strain BXN5-13(T) represents a novel species within the genus Phycicoccus, for which the name Phycicoccus ginsenosidimutans sp. nov. is proposed. The type strain is BXN5-13(T) (=KCTC 19419(T)=DSM 21006(T)=LMG 24462(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / classification*
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification*
  • Actinomycetales / physiology
  • Bacterial Typing Techniques
  • Base Composition
  • China
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Diaminopimelic Acid / analysis
  • Fatty Acids / analysis
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Panax
  • Phospholipids / analysis
  • Phylogeny
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Phospholipids
  • Quinones
  • RNA, Ribosomal, 16S
  • Diaminopimelic Acid

Associated data

  • GENBANK/EU332824