The genetic location of the self-incompatibility locus in white clover (Trifolium repens L.)

Theor Appl Genet. 2010 Aug;121(3):567-76. doi: 10.1007/s00122-010-1330-9. Epub 2010 Apr 10.

Abstract

White clover (Trifolium repens L.) is a forage legume of considerable economic importance in temperate agricultural systems. It has a strong self-incompatibility system. The molecular basis of self-incompatibility in T. repens is unknown, but it is under the control of a single locus, which is expressed gametophytically. To locate the self-incompatibility locus (S locus) in T. repens, we carried out cross-pollination experiments in an F(1) mapping population and constructed a genetic linkage map using amplified fragment length polymorphism and simple sequence repeat markers. As the first step in a map-based cloning strategy, we locate for the first time the S locus in T. repens on a genetic linkage map, on the homoeologous linkage group pair 1 (E), which is broadly syntenic to Medicago truncatula L. chromosome 1. On the basis of this syntenic relationship, the possibility that the S locus may or may not possess an S-RNase gene is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amplified Fragment Length Polymorphism Analysis
  • Chromosome Mapping*
  • Genetic Linkage / genetics*
  • Genetic Markers / genetics*
  • Microsatellite Repeats
  • Synteny
  • Trifolium / genetics*

Substances

  • Genetic Markers