Functional characterization of murine CD25 expressing B cells

Scand J Immunol. 2010 Apr;71(4):275-82. doi: 10.1111/j.1365-3083.2010.02380.x.

Abstract

B cells are an important part of both innate and adaptive immune system. Their ability to produce antibodies, cytokines and to present antigen makes them a crucial part in defence against pathogens. In this study, we have in naïve Naval Medical Research Institute mice functionally characterized a subpopulation of splenic B cells expressing CD25, which comprise about 1% of the total B cell compartment. Murine spleen cells were sorted into two highly purified B cell populations either CD19(+) CD25(+) or CD19(+) CD25(-). We found that CD25(+) B cells secreted higher levels of IL-6, IL-10 and INFgamma in response to different TLR-agonists, and were better at presenting alloantigen to CD4(+) T cells. CD25 expressing B cells spontaneously secreted immunoglobulins of IgA, IgG and IgM subclass and had better migratory ability when compared with CD25(-) B cells. In conclusion, our results demonstrate that CD25(+) B cells are highly activated and functionally mature. Therefore, we suggest that this population plays a major role in the immune system and may belong to the memory B-cell population.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen Presentation / immunology
  • B-Lymphocyte Subsets / immunology*
  • B-Lymphocytes / immunology*
  • Cell Movement
  • Cell Separation
  • Cytokines / biosynthesis
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Flow Cytometry
  • Interleukin-2 Receptor alpha Subunit / immunology*
  • Lymphocyte Activation / immunology
  • Lymphocyte Culture Test, Mixed
  • Mice
  • Mice, Inbred C57BL

Substances

  • Cytokines
  • Interleukin-2 Receptor alpha Subunit