Arabidopsis tiling array analysis to identify the stress-responsive genes

Methods Mol Biol. 2010:639:141-55. doi: 10.1007/978-1-60761-702-0_8.

Abstract

Plants respond and adapt to drought, cold, and high-salinity stresses. Stress-inducible gene products function in the stress response and tolerance in plants. Using cDNA microarrays and oligonucleotide microarrays, stress-inducible genes have been identified in various plant species so far. Recently, tiling array technology has become a powerful tool for the whole-genome transcriptome analysis. We applied the Arabidopsis Affymetrix tiling arrays to study the whole-genome transcriptome under drought, cold, and high-salinity stresses and identified a large number of drought, cold, and high-salinity stress-inducible genes and transcriptional units (TUs).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Biotin / metabolism
  • Computational Biology
  • DNA, Complementary / biosynthesis
  • Droughts
  • Gene Expression Regulation, Plant
  • Genes, Plant / genetics*
  • Oligonucleotide Array Sequence Analysis / methods*
  • RNA, Complementary / genetics
  • RNA, Plant / genetics
  • RNA, Plant / isolation & purification
  • Staining and Labeling
  • Stress, Physiological / genetics*
  • Transcription, Genetic

Substances

  • DNA, Complementary
  • RNA, Complementary
  • RNA, Plant
  • Biotin