Evaluation of different serological tests for the detection of antibodies against highly pathogenic avian influenza in experimentally infected ostriches (Struthio camelus)

Avian Pathol. 2010 Feb;39(1):11-5. doi: 10.1080/03079450903431390.


In the present study we collected 177 serum samples from ostriches (Struthio camelus) infected experimentally with A/ostrich/South Africa/Middleton/2004 (H5N2) highly pathogenic avian influenza virus. We tested these samples using the haemagglutination inhibition (HI) test, the agar gel immunodiffusion test and three enzyme-linked immunosorbent assay kits. We considered the HI test, with homologous antigen and including pre-treatment of sera with 10% chicken red blood cells, as the gold standard. Detectable specific antibodies appeared on day 7 post-infection and persisted until the termination of the experiment. The relative sensitivity and specificity of the tests under evaluation and Cohen's K value were calculated. The results reported herein could be of assistance to decision-makers in drafting guidelines for the definition of the health status of ostriches and for trade purposes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Antibodies, Viral / immunology
  • Enzyme-Linked Immunosorbent Assay / methods
  • Hemagglutination Inhibition Tests / methods
  • Immunodiffusion / methods
  • Influenza A Virus, H5N2 Subtype / immunology*
  • Influenza A Virus, H5N2 Subtype / isolation & purification
  • Influenza in Birds / blood
  • Influenza in Birds / diagnosis*
  • Influenza in Birds / immunology
  • Influenza in Birds / virology
  • Sensitivity and Specificity
  • Struthioniformes* / immunology
  • Struthioniformes* / virology


  • Antibodies, Viral