Rapid diagnosis of Old World Leishmaniasis by high-resolution melting analysis of the 7SL RNA gene

Abedelmajeed Nasereddin; Charles L Jaffe

doi:10.1128/JCM.00553-10

Rapid diagnosis of Old World Leishmaniasis by high-resolution melting analysis of the 7SL RNA gene

J Clin Microbiol. 2010 Jun;48(6):2240-2. doi: 10.1128/JCM.00553-10. Epub 2010 Apr 14.

Authors

Abedelmajeed Nasereddin¹, Charles L Jaffe

Affiliation

¹ Department of Microbiology and Molecular Genetics, IMIRC, the Kuvin Center for the Study of Infectious and Tropical Diseases, the Hebrew University-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel.

Abstract

High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World Leishmania was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (P < 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

DNA Primers / genetics
Humans
Leishmania / genetics*
Leishmania / isolation & purification
Leishmaniasis, Cutaneous / diagnosis*
Leishmaniasis, Cutaneous / parasitology
Parasitology / methods*
Polymerase Chain Reaction / methods*
RNA, Protozoan / genetics*
RNA, Small Cytoplasmic / genetics*
Sensitivity and Specificity
Signal Recognition Particle / genetics*
Transition Temperature*

Substances

7SL RNA
DNA Primers
RNA, Protozoan
RNA, Small Cytoplasmic
Signal Recognition Particle