Stevia rebaudiana Bertoni extract supplementation improves lipid and carnitine profiles in C57BL/6J mice fed a high-fat diet

J Sci Food Agric. 2010 May;90(7):1099-105. doi: 10.1002/jsfa.3906.


Background: Stevia (Stevia rebaudiana Bertoni) is a non-caloric natural-source alternative to artificially produced sugar substitutes. This study investigated the effect of stevia extract on lipid profiles in C57BL/6J mice. Forty mice were divided into four groups: N-C (normal diet and distilled water), H-C (high-fat diet and distilled water), H-SC (high fat diet and sucrose, 1 mL kg(-1) per day), and H-SV (high-fat diet and stevia extract, 1 mL kg(-1) per day).

Results: Body weight gain was significantly higher in the H-SC group than in the H-SV group. Triglyceride concentrations in serum and liver were lower in the H-SV group than in the H-SC group. Serum total cholesterol concentrations were lower in the H-SV and H-C groups compared to the H-SC group. The concentrations of acid-insoluble acylcarnitine (AIAC) in serum were higher in the H-SV group than in the H-C and H-SC groups and the acyl/free carnitine level in liver was significantly higher in the H-SV group than in the N-C group. These results were supported by mRNA expression of enzymes related to lipid metabolism (ACO, PPARalpha, ACS, CPT-I, ACC) assessed by real-time polymerase chain reaction.

Conclusion: These results suggest that the supplementation of stevia extract might have an anti-obesity effect on high-fat diet induced obese mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Weight / drug effects
  • Carnitine / blood
  • Carnitine / metabolism*
  • Dietary Fats / administration & dosage*
  • Dietary Sucrose / adverse effects*
  • Dietary Supplements
  • Enzymes / genetics
  • Enzymes / metabolism
  • Hypercholesterolemia / prevention & control
  • Lipid Metabolism
  • Lipids / blood*
  • Mice
  • Mice, Inbred C57BL
  • Obesity / prevention & control*
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stevia*
  • Sucrose / adverse effects
  • Weight Gain / drug effects


  • Dietary Fats
  • Dietary Sucrose
  • Enzymes
  • Lipids
  • Plant Extracts
  • RNA, Messenger
  • Sucrose
  • Carnitine