The anionic amphiphile SDS is an antagonist for the human neutrophil formyl peptide receptor 1

Biochem Pharmacol. 2010 Aug 1;80(3):389-95. doi: 10.1016/j.bcp.2010.04.009. Epub 2010 Apr 13.


The anionic amphiphil sodium dodecyl sulfate (SDS) is commonly used to activate the superoxide-generating NADPH-oxidase complex in cell-free systems, but very little is known about the effects of SDS on intact cells. It was, however, recently shown that SDS causes a translocation and an activation of Rac (a small G-protein) in intact cells, but this signal is not in its own sufficient to activate the oxidase (Nigorikawa et al. (2004) [1]). We found that SDS acted as an antagonist for FPR1, one of the neutrophil members of the formyl peptide receptor family. Accordingly, SDS reduced superoxide anion production induced by the chemoattractant formylmethionyl-leucyl-phenylalanine (fMLF). The receptor specificity of SDS was fairly high, but the concentration range in which it worked was narrow. The length of the carbohydrate chain as well as the charge of the molecule was of importance for the antagonistic effects. Signaling through FPR2, a closely related receptor also expressed in neutrophils, was not inhibited by SDS. On the contrary, the response induced by the FPR2-specific agonist WKYMVM was primed by SDS. The precise mechanism behind the primed state is not known, but might be related to the effects earlier described for SDS on the small G-protein Rac, that is of importance for a proper transduction of the down-stream signals from the occupied receptor.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anions
  • Dose-Response Relationship, Drug
  • Humans
  • Neutrophil Activation / drug effects
  • Neutrophil Activation / physiology
  • Neutrophils / drug effects*
  • Neutrophils / metabolism*
  • Protein Binding / drug effects
  • Protein Binding / physiology
  • Receptors, Formyl Peptide / antagonists & inhibitors*
  • Receptors, Formyl Peptide / metabolism
  • Sodium Dodecyl Sulfate / chemistry
  • Sodium Dodecyl Sulfate / pharmacology*


  • Anions
  • FPR1 protein, human
  • Receptors, Formyl Peptide
  • Sodium Dodecyl Sulfate