This case control study was done to study the seropositivity of helicobacter pylori among the fish handlers and to find out rapid urease test (RUT) & haematoxillin and eosin staining report correlation of seropositive cases. It was performed among fish handlers in the period of July 2005 to June 2006. Blood samples were collected from fish handlers (Group I) and some non-fish handler control subjects (Group II) to perform Anti-H. Pylori IgG test by ELISA method. Samples were collected from both the sea water area (Paikgacha, Khulna) and fresh water area (Derai, Sunamgonj and Dhaka). Seropositive cases having complaints and treatment history of peptic ulcer diseases were motivated for endoscopic examination to collect biopsy from upper GIT and rapid urease test (RUT) hematoxillin & eosin (H & E) staining were performed. A total of 235 respondents were included in this study, among them 163 were fish handlers (80 sea water and 83 fresh water) and 72 were non fish handler control (25 sea water, 22 fresh water from Sunamgonj, 25 fresh water from Dhaka city). Serum anti- H. pylori test was positive in 126(77.30%) cases in fish handler group (a) and 27(37.5%) cases in the control group (b). Seropositive cases were observed more among the higher age group (>40 years) than in lower age group (<40 years) which was 86% and 67% respectively. Endoscopic examination was done among the seropositive cases having positive PUD features. Total seropositive cases were 153 and among them 81 have positive PUD features, among them 48 cases were selected for endoscopic examination. Among the fish handlers out of 34 cases, 31(91.17%) were RUT positive and out of 14 non-fish handlers 6(42.85%) were RUT positive. Among the fish handlers, 28(82.35%) of the 34-biopsy specimens were H & E positive and among the non-fish handlers, out of 14 samples, 5(35.71%) were H & E positive. Fish handlers have more association of H. Pylori infection than non fish handlers and higher age group persons are more prone to H. Pylori infection. A significant number of seropositive persons have association with positive RUT and H & E staining report.