High-fat diet-induced neuropathy of prediabetes and obesity: effect of PMI-5011, an ethanolic extract of Artemisia dracunculus L

Mediators Inflamm. 2010;2010:268547. doi: 10.1155/2010/268547. Epub 2010 Apr 8.

Abstract

Artemisia species are a rich source of herbal remedies with antioxidant and anti-inflammatory properties. We evaluated PMI-5011, an ethanolic extract of Artemisia dracunculus L., on neuropathy in high-fat diet-fed mice, a model of prediabetes and obesity developing oxidative stress and proinflammatory changes in peripheral nervous system. C57Bl6/J mice fed high-fat diet for 16 weeks developed obesity, moderate nonfasting hyperglycemia, nerve conduction deficit, thermal and mechanical hypoalgesia, and tactile allodynia. They displayed 12/15-lipoxygenase overexpression, 12(S)-hydroxyeicosatetraenoic acid accumulation, and nitrosative stress in peripheral nerve and spinal cord. PMI-5011 (500 mg kg(-1) d(-1), 7 weeks) normalized glycemia, alleviated nerve conduction slowing and sensory neuropathy, and reduced 12/15-lipoxygenase upregulation and nitrated protein expression in peripheral nervous system. PMI-5011, a safe and nontoxic botanical extract, may find use in treatment of neuropathic changes at the earliest stage of disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Artemisia / chemistry*
  • Behavior, Animal / physiology
  • Blood Glucose / metabolism
  • Diabetic Neuropathies* / drug therapy
  • Diabetic Neuropathies* / pathology
  • Dietary Fats*
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Obesity* / complications
  • Obesity* / drug therapy
  • Pain Measurement
  • Plant Extracts / therapeutic use*
  • Prediabetic State* / complications
  • Prediabetic State* / drug therapy

Substances

  • Blood Glucose
  • Dietary Fats
  • PMI-5011
  • Plant Extracts