Multi-factorial modulation of IGD motogenic potential in MSF (migration stimulating factor)

Exp Cell Res. 2010 Sep 10;316(15):2465-76. doi: 10.1016/j.yexcr.2010.04.003. Epub 2010 Apr 18.


Migration Stimulating Factor (MSF) is a genetically truncated isoform of fibronectin (Fn). MSF is a potent stimulator of fibroblast migration, whereas full length Fn is devoid of motogenic activity. MSF and Fn contain four IGD motifs, located in the 3rd, 5th, 7th and 9th type I modules; these modules are referred to as (3)FnI, (5)FnI, (7)FnI and (9)FnI, respectively. We have previously reported that mutation of IGD motifs in modules (7)FnI and (9)FnI of MSF is sufficient to completely abolish the motogenic response of target adult skin fibroblasts. We now report that the IGD sequences in (3)FnI and (5)FnI are also capable of exhibiting motogenic activity when present within fragments of MSF. When present within (1-5)FnI, these sequences require the presence of serum or vitronectin for their motogenic activity to be manifest, whereas the IGD sequences in (7)FnI and (9)FnI are bioactive in the absence of serum factors. All MSF and IGD-containing peptides stimulated the phosphorylation of the integrin binding protein focal adhesion kinase (FAK) but did not necessarily affect migration. These results suggest that steric hindrance determines the motogenic activity of MSF and Fn, and that both molecules contain cryptic bioactive fragments.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Motifs / physiology
  • Amino Acid Sequence
  • Animals
  • Cell Movement / drug effects
  • Cell Movement / physiology*
  • Cells, Cultured
  • Cytokines / chemistry*
  • Cytokines / pharmacology
  • Cytokines / physiology*
  • Dose-Response Relationship, Drug
  • Drosophila melanogaster
  • Fibroblasts / drug effects
  • Fibroblasts / physiology
  • Fibronectins / chemistry
  • Fibronectins / physiology
  • Humans
  • Male
  • Molecular Motor Proteins / chemistry
  • Molecular Motor Proteins / physiology
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Peptide Fragments / pharmacology
  • Rats
  • Sequence Homology, Amino Acid


  • Cytokines
  • FN1 protein, human
  • Fibronectins
  • Molecular Motor Proteins
  • Peptide Fragments