A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies

Hongyan Xia; Lihong Liu; Ann Nordengrahn; István Kiss; Malik Merza; Ronnie Eriksson; Jonas Blomberg; Sándor Belák

doi:10.1016/j.jviromet.2010.04.009

A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies

J Virol Methods. 2010 Sep;168(1-2):18-21. doi: 10.1016/j.jviromet.2010.04.009. Epub 2010 Apr 18.

Authors

Hongyan Xia¹, Lihong Liu, Ann Nordengrahn, István Kiss, Malik Merza, Ronnie Eriksson, Jonas Blomberg, Sándor Belák

Affiliation

¹ Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.

Abstract

This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Viral / blood*
Bovine Virus Diarrhea-Mucosal Disease / diagnosis*
Cattle
Diarrhea Viruses, Bovine Viral / immunology
Diarrhea Viruses, Bovine Viral / isolation & purification*
Immunoassay / methods
Microspheres
Reproducibility of Results
Sensitivity and Specificity
Virology / methods*

Substances

Antibodies, Viral