Sporamin induce apoptosis in human tongue carcinoma cells by down-regulating Akt/GSK-3 signaling

Fundam Clin Pharmacol. 2011 Apr;25(2):229-36. doi: 10.1111/j.1472-8206.2010.00830.x.

Abstract

We investigated the effects of sporamin, the major soluble protein with a kunitz-type trypsin inhibitory activity in the root tuber of the sweet potato, on cell proliferation, apoptosis, Akt/GSK-3 signaling and its related genes to provide more insights in the mechanism behind the inhibitory effects of sporamin in a human tongue cancer line Tca8113. In this study, sporamin inhibited cell proliferation and induced apoptosis in Tca8113 cells in a concentration-dependent and time-dependent manner. Consistently, Bax was up-regulated and Bcl-2 was down-regulated in sporamin-treated cells. Furthermore, Akt/GSK-3 signaling was down-regulated in sporamin-treated cells. Consistently, the phosphorylated Bad was significantly declined in sporamin-treated Tca8113 cells. These results suggest the antiproliferative effects of sporamin in Tca8113 cells might result partly from induction of apoptosis by down-regulating Akt/GSK-3 pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / administration & dosage
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Dose-Response Relationship, Drug
  • Down-Regulation / drug effects
  • Glycogen Synthase Kinase 3 / drug effects
  • Glycogen Synthase Kinase 3 / metabolism
  • Humans
  • Phosphorylation / drug effects
  • Plant Proteins / administration & dosage
  • Plant Proteins / pharmacology*
  • Proto-Oncogene Proteins c-akt / drug effects
  • Proto-Oncogene Proteins c-akt / metabolism
  • Signal Transduction / drug effects
  • Tongue Neoplasms / drug therapy*
  • Tongue Neoplasms / pathology

Substances

  • Antineoplastic Agents
  • Plant Proteins
  • sporamin protein, Ipomoea batatas
  • Proto-Oncogene Proteins c-akt
  • Glycogen Synthase Kinase 3