Effect of POLY-MVA, a palladium alpha-lipoic acid complex formulation against declined mitochondrial antioxidant status in the myocardium of aged rats

Food Chem Toxicol. 2010 Jul;48(7):1858-62. doi: 10.1016/j.fct.2010.04.022. Epub 2010 Apr 20.

Abstract

Palladium alpha-lipoic acid formulation--'POLY-MVA' is found to enhance the activities of Krebs cycle dehydrogenases and respiratory complexes in the heart of aged rat. In this study, we aimed to evaluate the effect of POLY-MVA on the activities of antioxidant status in the heart mitochondria of aged rat. We determined the activities of manganese-superoxide dismutase (Mn SOD), catalase (CAT), glutathione peroxidase (GPx), and level of reduced GSH and lipid peroxidation in the heart mitochondria of aged rats, after administering POLY-MVA (0.05 ml/kg; equivalent to 0.38 mg complexed alpha-lipoic acid/kg) orally once daily for 30 days. DL-alpha-lipoic acid (0.38 mg/kg, p.o) treated for 30 days was kept as the positive control. We found that the antioxidant in the aged control was declined significantly than the young control. The formulation significantly (p<0.05) enhanced the activity of CAT and GPx compared to the aged control. The level of GSH was also significantly improved and the level of lipid peroxidation was decreased significantly (p<0.05) by POLY-MVA. The results indicate that POLY-MVA is effective to protect the age-linked decline of myocardial mitochondrial antioxidant status. The findings suggest the use of this formulation against myocardial aging.

MeSH terms

  • Aging / physiology*
  • Animals
  • Antioxidants / metabolism*
  • Antioxidants / pharmacology*
  • Catalase / metabolism
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • In Vitro Techniques
  • Lipid Peroxidation / drug effects
  • Male
  • Mitochondria, Heart / drug effects*
  • Myocardium / metabolism*
  • Palladium / pharmacology*
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / metabolism
  • Thioctic Acid / pharmacology*

Substances

  • Antioxidants
  • Palladium
  • Thioctic Acid
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione