Evaluation of hepcidin isoforms in hemodialysis patients by a proteomic approach based on SELDI-TOF MS

J Biomed Biotechnol. 2010;2010:329646. doi: 10.1155/2010/329646. Epub 2010 Apr 15.

Abstract

The hepatic iron regulator hormone hepcidin consists, in its mature form, of 25 amino acids, but two other isoforms, hepcidin-20 and hepcidin-22, have been reported, whose biological meaning remains poorly understood. We evaluated hepcidin isoforms in sera from 57 control and 54 chronic haemodialysis patients using a quantitative proteomic approach based on SELDI-TOF-MS. Patients had elevated serum levels of both hepcidin-25 and hepcidin-20 as compared to controls (geometric means: 7.52 versus 4.69 nM, and 4.06 versus 1.76 nM, resp., P < .05 for both). The clearance effects of a single dialysis session by different dialysis techniques and membranes were also investigated, showing an average reduction by 51.3% +/- 29.2% for hepcidin-25 and 34.2% +/- 28.4% for hepcidin-20 but only minor differences among the different dialysis modalities. Measurement of hepcidin isoforms through MS-based techniques can be a useful tool for better understanding of their biological role in hemodialysis patients and other clinical conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antimicrobial Cationic Peptides / blood*
  • Case-Control Studies
  • Female
  • Hepcidins
  • Humans
  • Linear Models
  • Male
  • Middle Aged
  • Peptide Fragments / blood*
  • Protein Isoforms
  • Proteomics / methods
  • Renal Dialysis / methods
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Antimicrobial Cationic Peptides
  • Hepcidins
  • Peptide Fragments
  • Protein Isoforms
  • hepcidin (6-25), human
  • hepcidin 25, human