Adulticidal activity of essential oil of Lantana camara leaves against mosquitoes

Indian J Med Res. 2010 Mar;131:434-9.


Background & objectives: Development of insect resistance to synthetic pesticides, high operational cost and environmental pollution have created the need for developing alternative approaches to control vector-borne diseases. In the present study we have investigated the insecticidal activity of essential oil isolated from the leaves of Lantana camara against mosquito vectors.

Methods: Essential oil was isolated from the leaves of L. camara using hydro-distillation method. Bioassay test was carried out by WHO method for determination of adulticidal activity against mosquitoes. Different compounds were identified by gas chromatography-mass spectrometry analysis.

Results: LD(50) values of the oil were 0.06, 0.05, 0.05, 0.05 and 0.06 mg/cm(2) while LD(90) values were 0.10, 0.10, 0.09, 0.09 and 0.10 mg/cm(2) against Ae. aegypti, Cx. quinquefasciatus, An. culicifacies, An. fluvialitis and An. stephensi respectively. KDT(50) of the oil were 20, 18, 15, 12, and 14 min and KDT(90) values were 35, 28 25, 18, 23 min against Ae. aegypti, Cx. quinquefasciatus, An. culicifacies, An. fluviatilis and An. stephensi, respectively on 0.208 mg/cm(2) impregnated paper. Studies on persistence of essential oil of L. camara on impregnated paper revealed that it has more adulticidal activity for longer period at low storage temperature. Gas chromatographic-mass spectrometric analysis of essential oil showed 45 peaks. Caryophyllene (16.37%), eucalyptol (10.75%), alpha-humelene (8.22%) and germacrene (7.41%) were present in major amounts and contributed 42.75 per cent of the total constituents.

Interpretation & conclusions: Essential oil from the leaves of L. camara possesses adulticidal activity against different mosquito species that could be utilized for development of oil-based insecticide as supplementary to synthetic insecticides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Assay
  • Culicidae / drug effects*
  • Drug Evaluation, Preclinical / methods
  • Female
  • Gas Chromatography-Mass Spectrometry / methods
  • Insect Repellents / pharmacology
  • Lantana / metabolism*
  • Mosquito Control / methods*
  • Oils, Volatile / metabolism
  • Pesticides / pharmacology
  • Plant Extracts / pharmacology
  • Plant Leaves / metabolism*
  • Temperature


  • Insect Repellents
  • Oils, Volatile
  • Pesticides
  • Plant Extracts