Targeted Gene Deletions in C. Elegans Using Transposon Excision

Nat Methods. 2010 Jun;7(6):451-3. doi: 10.1038/nmeth.1454. Epub 2010 Apr 25.

Abstract

We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics*
  • Comparative Genomic Hybridization
  • DNA Repair
  • DNA Transposable Elements / genetics*
  • DNA-Binding Proteins / physiology
  • Gene Deletion*
  • Transposases / physiology

Substances

  • DNA Transposable Elements
  • DNA-Binding Proteins
  • mariner transposases
  • Transposases